42:315–324 18654Įl Kinany S, Achbani E, Faggroud M et al (2018) Effect of organic fertilizer and commercial arbuscular mycorrhizal fungi on the growth of micropropagated date palm cv. Įhlers R, Kracht L, Witte J (1989) Case history of horizontal wells drilled with navigation technology in European operations. Įddings MA, Johnson MA, Gale BK (2008) Determining the optimal PDMS – PDMS bonding technique for microfluidic devices. ĭouds DD, Nagahashi G, Hepperly PR (2010) On-farm production of inoculum of indigenous arbuscular mycorrhizal fungi and assessment of diluents of compost for inoculum production. Published by Elsevier Incĭijkshoorn JP, Schutyser MAI, Wagterveld RM, Schroën CGPH, Boom RM (2017) A comparison of microfiltration and inertia-based microfluidics for large scale suspension separation. īushnell T, Trotter J (2014) Inside the flow cytometer: hardware. īecker A, Meister A, Wilhelm C (2002) Flow cytometric discrimination of various phycobilin-containing phytoplankton groups in a hypertrophic reservoir. Īmigoni L, Colombo S, Alberghina L, Martegani E (2010) Towards a yeast cell cycle hybrid model: a new experimental model to study the link between PKA activity and cell cycle in budding yeast. Īmaya-Carpio L, Davies FT, Fox T, He C (2009) Arbuscular mycorrhizal fungi and organic fertilizer influence photosynthesis, root phosphatase activity, nutrition, and growth of Ipomoea carnea ssp. Īllen MF, Moore TS, Christensen M, Stanton N (1979) Growth of vesicular-arbuscular-mycorrhizal and nonmycorrhizal bouteloua gracilis in a defined medium. Īkhtar MS, Siddiqui ZA, Wiemken A (2011) Alternative farming systems, biotechnology, drought stress and ecological fertilisation:263–292. Microbes Microb Technol Agric Environ Appl:1–516. Using the device, spore purity, the percentage of spore numbers against the total number of particles counted in the collecting chamber reached 96.62% (median, n = 10) which is greater than the spore purity obtained from the conventional method (88.89% (median, n = 10)).Īhmad I, Ahmad F, Pichtel J (2011) Microbes and microbial technology: agricultural and environmental applications. The targeted spore flows in a stream to the collection chamber via temporary cross flow. Users can press a switch to generate MTFD when the spore arrives at the selection site.
The device is a single spore streamer equipped with a manual temporary flow diversion (MTFD) mechanism to select single spores. Here, we developed a microfluidic device to aid manual separation of spores from a filtered and centrifuged suspension. The conventional procedure is skill-demanding, labor-intensive, and time-consuming. Filtered and centrifuged spores have to be hand-picked under a microscope. The acquisition of isolated spores is a required step in these studies however, spore quality control and spore separation are bottlenecks. Studies of the biology and utilization of these fungi are key to their successful use in the biofertilizer industry. Arbuscular mycorrhizal fungi are beneficial components often included in biofertilizers.